{"product_id":"pcr-biosystems-isofast-bst-1-step-mix","title":"PCR Biosystems IsoFast Bst 1-Step Mix","description":"\u003ch4\u003e\u003cstrong\u003eIsoFast Bst 1-Step Mix\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eIsoFast® Bst 1-Step Mix is a versatile isothermal amplification reagent designed for rapid and sensitive one-step reverse transcription and DNA amplification of RNA targets. It combines strand-displacing IsoFast Bst DNA Polymerase with a highly active modified MMLV Reverse Transcriptase (RTase Go) in a 2x master mix format, enabling efficient nucleic acid amplification at a constant temperature of 65°C.\u003c\/p\u003e\n\u003cp\u003eThe product line includes multiple pack sizes and formats suitable for real-time or end-point detection in molecular diagnostics, infectious disease detection, and research applications such as RT-LAMP.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Details:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDual enzyme system: IsoFast Bst strand-displacing polymerase and modified MMLV RTase\u003c\/li\u003e\n\u003cli\u003e2x master mix with integrated enzymes, buffer, fluorescent dye, and RNase inhibitor\u003c\/li\u003e\n\u003cli\u003eIsothermal amplification protocol at 65°C, typically completed in 30 minutes\u003c\/li\u003e\n\u003cli\u003eFluorescence-based real-time detection compatibility\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eOptimized for robust performance across diverse nucleic acid templates and sample types\u003c\/li\u003e\n\u003cli\u003eValidated for SARS-CoV-2 detection and other viral RNA targets\u003c\/li\u003e\n\u003cli\u003eStorage temperature: -20°C\u003c\/li\u003e\n\u003cli\u003eManufacturer: PCR Biosystems\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRapid and highly sensitive detection of RNA targets in a single step\u003c\/li\u003e\n\u003cli\u003eEnhanced strand displacement for efficient amplification\u003c\/li\u003e\n\u003cli\u003eRobust amplification even in presence of inhibitors\u003c\/li\u003e\n\u003cli\u003eCompatible with a variety of real-time PCR and isothermal platforms\u003c\/li\u003e\n\u003cli\u003eReliable enzyme mix designed for routine molecular biology and diagnostic use\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cbr\u003e\u003c\/h4\u003e","brand":"PCR Biosystems","offers":[{"title":"25 µL \/ Pack of 100 \/ New - Unopened (Sealed) \/ 01-03-2026","offer_id":52437819523399,"sku":"PB80.21-01","price":0.0,"currency_code":"USD","in_stock":false},{"title":"25 µL \/ Pack of 500 \/ New - Unopened (Sealed) \/ 01-03-2026","offer_id":52442728923463,"sku":"PB80.21-05","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0802\/7245\/8055\/files\/convert_68daf0ad-6c2d-48b2-a1b7-e4c91e668e15.png?v=1759158960","url":"https:\/\/checkout.wasteless.bio\/en-us\/products\/pcr-biosystems-isofast-bst-1-step-mix","provider":"Wasteless Bio","version":"1.0","type":"link"}