{"product_id":"pcr-biosystems-isofast-bst-polymerase","title":"PCR Biosystems IsoFast Bst Polymerase","description":"\u003ch4\u003e\u003cstrong\u003eIsoFast Bst Polymerase\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003eDescription:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIsoFast® Bst Polymerase is a DNA polymerase with significant strand displacement activity, making it ideal for various nucleic acid amplification techniques. It is particularly well-suited for whole genome amplification, multiple displacement amplification, and isothermal amplification methods. The enzyme is a recombinant form of the large fragment of Bst DNA polymerase, engineered to retain 5'-3' polymerase activity while lacking 5'-3' exonuclease activity. It performs optimally at 65°C but demonstrates functionality across a broad temperature range (55°C-70°C) and is inactivated at 80°C.\u003c\/p\u003e\n\u003cp\u003eThe IsoFast® Hot Start Bst Polymerase \u0026amp; Mixes variant integrates AptaLock™ hot start technology, ensuring superior specificity and sensitivity for isothermal amplification workflows, LAMP applications, and point-of-care testing scenarios. This version minimizes primer dimer formation and non-specific target amplification.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRobust strand displacement capability\u003c\/li\u003e\n\u003cli\u003eEfficient for isothermal amplification processes\u003c\/li\u003e\n\u003cli\u003eAptaLock™ hot start technology for enhanced precision (Hot Start variant)\u003c\/li\u003e\n\u003cli\u003eVersatile format options, including ready-to-use mixes and fluorescent dye integration\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eWhole genome amplification\u003c\/li\u003e\n\u003cli\u003eMultiple displacement amplification\u003c\/li\u003e\n\u003cli\u003eIsothermal amplification\u003c\/li\u003e\n\u003cli\u003eLoop-mediated isothermal amplification (LAMP)\u003c\/li\u003e\n\u003cli\u003eMolecular diagnostics\u003c\/li\u003e\n\u003cli\u003ePoint-of-care testing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTechnical Specifications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme Type:\u003c\/strong\u003e DNA polymerase\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKey Activity:\u003c\/strong\u003e Strand displacement\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimal Temperature:\u003c\/strong\u003e 65°C (functional range: 55°C-70°C)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHeat Inactivation:\u003c\/strong\u003e 80°C\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExonuclease Activity:\u003c\/strong\u003e None (5'-3')\u003cstrong\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTotal Reaction Volume:\u003c\/strong\u003e 25 µl\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage Temperature:\u003c\/strong\u003e -20°C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e PCR Biosystems\u003c\/p\u003e","brand":"PCR Biosystems","offers":[{"title":"Fluorescent Dye \/ 25 µL \/ Pack of 200 Reactions \/ New - Unopened (Sealed) \/ 01-03-2026","offer_id":52530992546119,"sku":"PB80.30-02","price":0.0,"currency_code":"USD","in_stock":false},{"title":"Bst Polymerase \/ Pack of 1600 Units \/ New - Unopened (Sealed) \/ 01-03-2026","offer_id":52930594242887,"sku":"PB80.10-01","price":0.0,"currency_code":"USD","in_stock":false},{"title":"Bst Polymerase \/ Pack of 8000 Units \/ New - Unopened (Sealed) \/ 01-03-2026","offer_id":52930594275655,"sku":"PB80.10-08","price":0.0,"currency_code":"USD","in_stock":false},{"title":"Bst Mix \/ 25 µL \/ Pack of 100 Reactions \/ New - Unopened (Sealed) \/ 01-03-2026","offer_id":52930594308423,"sku":"PB80.12-01","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0802\/7245\/8055\/files\/convert_99fdfc3b-ce16-4d59-a1a2-93963ea8d97e.png?v=1759158953","url":"https:\/\/checkout.wasteless.bio\/en-us\/products\/pcr-biosystems-isofast-bst-polymerase","provider":"Wasteless Bio","version":"1.0","type":"link"}