{"product_id":"rd-systems-substrate-reagent-pack","title":"R\u0026D Systems Substrate Reagent Pack","description":"\u003cheader class=\"wlb-header\"\u003e\n\u003ch2 class=\"wlb-title\"\u003eSubstrate Reagent Pack\u003c\/h2\u003e\n\u003cp class=\"wlb-meta\"\u003eby\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"wlb-brand\"\u003eR\u0026amp;D Systems\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e· SKU\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"wlb-sku\"\u003eDY999\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/header\u003e\n\u003csection class=\"wlb-overview\"\u003e\n\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Substrate Reagent Pack (Catalog # DY999) from R\u0026amp;D Systems is a set of chromogenic ELISA substrate solutions formulated to produce a blue color in the presence of horseradish peroxidase (HRP)-conjugated detection reagents and, upon acid stop, a yellow signal measurable at ~450 nm. The pack includes paired reagents that are mixed immediately prior to use to support color development in immunoassays and other HRP-based detection formats. This reagent pack is intended for research use in sandwich ELISAs and similar assays.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"wlb-highlights\"\u003e\n\u003ch2\u003eHighlights\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIncludes multiple vials of matched substrate reagents for ELISA colour development\u003c\/li\u003e\n\u003cli\u003eOptimised for use with HRP-conjugated detection systems\u003c\/li\u003e\n\u003cli\u003eGenerates a measurable chromogenic signal at ~450 nm upon reaction and stopping\u003c\/li\u003e\n\u003cli\u003eReady to mix and apply to microplate wells for consistent development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection class=\"wlb-ataglance\"\u003e\n\u003ch2\u003eAt-a-glance\u003c\/h2\u003e\n\u003cdl\u003e\n\u003cdt\u003eKit name\u003c\/dt\u003e\n\u003cdd\u003eSubstrate Reagent Pack\u003c\/dd\u003e\n\u003cdt\u003eContents\u003c\/dt\u003e\n\u003cdd\u003e8 vials Colour Reagent A (stabilised hydrogen peroxide) and 8 vials Colour Reagent B (stabilised tetramethylbenzidine), ~12 mL\/vial\u003c\/dd\u003e\n\u003cdt\u003eApplication\u003c\/dt\u003e\n\u003cdd\u003eELISA and HRP-based immunoassays\u003c\/dd\u003e\n\u003cdt\u003eDetection\u003c\/dt\u003e\n\u003cdd\u003eChromogenic colour development ~450 nm\u003c\/dd\u003e\n\u003cdt\u003eUsage\u003c\/dt\u003e\n\u003cdd\u003eReagents mixed 1:1 and added to wells for colour development\u003c\/dd\u003e\n\u003cdt\u003eCondition\u003c\/dt\u003e\n\u003cdd\u003eNEW — Unused reagent pack\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003c\/section\u003e\n\u003csection class=\"wlb-applications\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis reagent pack is used in sandwich ELISAs and other immunoassays that employ HRP-conjugated detection antibodies or streptavidin-HRP to generate a colourimetric signal. Upon addition to assay wells and timed incubation, the TMB substrate system produces a quantifiable signal proportional to analyte concentration, suitable for research workflows in cytokine, growth factor, biomarker and protein quantification studies.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"wlb-sustainability\"\u003e\n\u003ch2\u003eSustainability\u003c\/h2\u003e\n\u003cp\u003eBy rehoming surplus lab products, this listing supports waste reduction and resource efficiency.\u003c\/p\u003e\n\u003c\/section\u003e","brand":"R\u0026D Systems","offers":[{"title":"Pack of 16 \/ New - Unopened (Sealed)","offer_id":52948527874375,"sku":"DY999","price":67.0,"currency_code":"USD","in_stock":false}],"url":"https:\/\/checkout.wasteless.bio\/en-us\/products\/rd-systems-substrate-reagent-pack","provider":"Wasteless Bio","version":"1.0","type":"link"}