{"product_id":"gibco-expifectamine-293-transfection-kit","title":"Gibco ExpiFectamine 293 Transfection Kit","description":"\u003cheader class=\"wlb-header\"\u003e\n\u003ch2 class=\"wlb-title\"\u003eExpiFectamine™ 293 Transfection Kit, For 1 L Culture\u003c\/h2\u003e\n\u003cp class=\"wlb-meta\"\u003eby\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"wlb-brand\"\u003eThermo Scientific\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e· SKU\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"wlb-sku\"\u003eA14524\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/header\u003e\n\u003csection class=\"wlb-overview\"\u003e\n\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe ExpiFectamine™ 293 Transfection Kit is a lipid-based transient transfection reagent system designed for high-efficiency delivery of plasmid DNA into Expi293F™ human embryonic kidney cells cultured at high density in suspension. The kit combines a cationic lipid-based ExpiFectamine™ 293 Reagent with two proprietary transfection enhancers formulated to maximise protein expression yields in Expi293 Expression System workflows. The protocol parallels standard transient transfection methods while delivering enhanced performance in high-density cultures.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"wlb-highlights\"\u003e\n\u003ch2\u003eHighlights\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIncludes ExpiFectamine™ 293 Reagent plus Transfection Enhancers 1 and 2 for boosted expression performance.\u003c\/li\u003e\n\u003cli\u003eOptimised for transient transfection of high-density Expi293F™ suspension cultures.\u003c\/li\u003e\n\u003cli\u003eAchieves protein yields 2- to 10-fold higher than many conventional transfection reagents in similar systems.\u003c\/li\u003e\n\u003cli\u003eApplicable from small to large culture volumes with scalable performance.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection class=\"wlb-ataglance\"\u003e\n\u003ch2\u003eAt-a-glance\u003c\/h2\u003e\n\u003cdl\u003e\n\u003cdt\u003eKit type\u003c\/dt\u003e\n\u003cdd\u003eTransient transfection reagent system\u003c\/dd\u003e\n\u003cdt\u003eDesigned for\u003c\/dt\u003e\n\u003cdd\u003eExpi293F™ suspension-adapted human embryonic kidney cells\u003c\/dd\u003e\n\u003cdt\u003eTechnique\u003c\/dt\u003e\n\u003cdd\u003eLipid-mediated transient transfection\u003c\/dd\u003e\n\u003cdt\u003eContents\u003c\/dt\u003e\n\u003cdd\u003e2.7 mL ExpiFectamine™ 293 Reagent (3×0.9 mL), 5 mL Transfection Enhancer 1, 50 mL Transfection Enhancer 2.\u003c\/dd\u003e\n\u003cdt\u003eCulture volume\u003c\/dt\u003e\n\u003cdd\u003eSufficient for transfection of ~1 L of culture.\u003c\/dd\u003e\n\u003cdt\u003eCompatible samples\u003c\/dt\u003e\n\u003cdd\u003ePlasmid DNA with Expi293F™ cultures.\u003c\/dd\u003e\n\u003cdt\u003eStorage\u003c\/dt\u003e\n\u003cdd\u003e2 °C – 8 °C (refrigerated).\u003c\/dd\u003e\n\u003cdt\u003eCondition\u003c\/dt\u003e\n\u003cdd\u003eNEW — Unused reagent kit\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003c\/section\u003e\n\u003csection class=\"wlb-applications\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis kit is used for transient gene delivery in high-density Expi293F™ suspension cultures to support recombinant protein expression, functional protein production and other molecular biology workflows where high yields of expressed protein are desired. It is suited to research applications in protein engineering, antibody production and expression optimisation studies using standard microbiology and cell culture equipment.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"wlb-sustainability\"\u003e\n\u003ch2\u003eSustainability\u003c\/h2\u003e\n\u003cp\u003eBy rehoming surplus lab products, this listing supports waste reduction and resource efficiency.\u003c\/p\u003e\n\u003c\/section\u003e","brand":"Thermo Fisher Scientific","offers":[{"title":"HEK 293 \/ Expi293 Expression System \/ 1 L \/ Unit \/ New - Unopened (Sealed)","offer_id":52949427716423,"sku":"A14524","price":50.21,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0802\/7245\/8055\/files\/convert_08b97918-6653-416a-b876-b819d1edf3fb.png?v=1765553697","url":"https:\/\/checkout.wasteless.bio\/products\/gibco-expifectamine-293-transfection-kit","provider":"Wasteless Bio","version":"1.0","type":"link"}